Tyrosine decarboxylase from Lactobacillus brevis: soluble expression and characterization.

The mechanism of the tyrosine transporter TyrP supports a proton motive tyrosine decarboxylation pathway in Lactobacillus brevis.

The tyrosine decarboxylase operon of Lactobacillus brevis IOEB9809 contains, adjacent to the tyrosine decarboxylase gene, a gene for TyrP, a putative tyrosine transporter. The two genes potentially form a proton motive tyrosine decarboxylation pathway. The putative tyrosine transporter gene of L. brevis was expressed in Lactococcus lactis and functionally characterized using right-side-out memb...

1 2 3 Gene cloning , expression , and characterization of phenolic acid 4 decarboxylase from Lactobacillus brevis RM 84 5

The effect of Lactobacillus brevis on Apoptosis and casp (casp8, casp3) gene Expression in HeLa Cancer Cells

Background and Aim: Cervical and breast cancer are considered to be the most common cause of death among Iranian women. Various studies have shown that probiotics are effective fighting cancer. The aim of the present study was to investigate the effect of Lactobacillus brevis on apoptosis and casp (casp3, casp8) gene expression in HeLa cancer cells.  Materials and Methods: In this study, L. br...

Agmatine deiminase pathway genes in Lactobacillus brevis are linked to the tyrosine decarboxylation operon in a putative acid resistance locus.

In lactic acid bacteria (LAB), amino acids and their derivatives may be converted into amine-containing compounds designated biogenic amines, in pathways providing metabolic energy and/or acid resistance to the bacteria. In a previous study, a pathway converting tyrosine to tyramine was detected in Lactobacillus brevis and a fragment of a gene possibly involved in the production of another biog...

Glutamate decarboxylase from Lactobacillus brevis: activation by ammonium sulfate.

In this study, the glutamate decarboxylase (GAD) gene from Lactobacillus brevis IFO12005 (Biosci. Biotechnol. Biochem., 61, 1168-1171 (1997)), was cloned and expressed. The deduced amino acid sequence showed 99.6% and 53.1% identity with GAD of L. brevis ATCC367 and L. lactis respectively. The His-tagged recombinant GAD showed an optimum pH of 4.5-5.0, and 54 kDa on SDS-PAGE. The GAD activity a...